Title

Application of phase correlation to the montage synthesis and three-dimensional reconstruction of large tissue volumes from confocal laser scanning microscopy

Document Type

Article

Publication Date

4-1-2006

Abstract

We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce "tiles" of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated. © Microscopy Society of America 2006.

Publication Name

Microscopy and Microanalysis

Volume Number

12

First Page

106

Last Page

112

Issue Number

2

DOI

10.1017/S143192760606017X

This document is currently not available here.

Share

COinS