Title

Ionic mechanisms of histamine-induced responses in guinea pig intracardiac neurons

Document Type

Article

Publication Date

1-1-2006

Abstract

Histamine, released from mast cells, can modulate the activity of intrinsic neurons in the guinea pig cardiac plexus. The present study examined the ionic mechanisms underlying the histamine-induced responses in these cells. Histamine evokes a small membrane depolarization and an increase in neuronal excitability. Using intracellular voltage recording from individual intracardiac neurons, we were able to demonstrate that removal of extracellular sodium reduced the membrane depolarization, whereas inhibition of K channels by 1 mM Ba , 2 mM Cs , or 5 mM tetraethylammonium had no effect. The depolarization was also not inhibited by either 10 μM Gd or a reduced Cl solution. The histamine-induced increase in excitability was unaffected by K channel inhibitors; however, it was reduced by either blockage of voltage-gated Ca channels with 200 μM Cd or replacement of extracellular Ca with Mg . Conversely, alterations in intracellular calcium with thapsigargin or caffeine did not inhibit the histamine-induced effects. However, in cells treated with both thapsigargin and caffeine to deplete internal calcium stores, the histamine-induced increase in excitability was decreased. Treatment with the phospholipase C inhibitor U73122 also prevented both the depolarization and the increase in excitability. From these data, we conclude that histamine, via activation of H receptors, activates phospholipase C, which results in 1) the opening of a nonspecific cation channel, such as a transient receptor potential channel 4 or 5; and 2) in combination with either the influx of Ca through voltage-gated channels or the release of internal calcium stores leads to an increase in excitability. Copyright © 2006 the American Physiological Society. + 2+ + 3+ - + 2+ 2+ 2+ 2+ 2+ 1

Publication Name

American Journal of Physiology - Regulatory Integrative and Comparative Physiology

Volume Number

290

Issue Number

1

DOI

10.1152/ajpregu.00498.2005

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