Title

Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

Document Type

Conference Proceeding

Publication Date

10-27-2004

Abstract

Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.

Publication Name

Proceedings of SPIE - The International Society for Optical Engineering

Volume Number

5370 III

First Page

1972

Last Page

1979

DOI

10.1117/12.536232

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