Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy
Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.
Proceedings of SPIE - The International Society for Optical Engineering
Slamani, M. A.; Krol, A.; Beaumont, J.; Price, R. L.; Coman, I. L.; and Lipson, E. D., "Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy" (2004). Faculty Articles Indexed in Scopus. 2002.