School

School of Humanities and Sciences

Department

Biology

ICC Theme

Inquiry, Imagination, and Innovation

Date

2-4-2019 8:50 AM

Abstract

The ability to regenerate is of high variability among animals. Model organisms such as the zebrafish (Danio rerio ) are exemplary in their ability to grow back limbs and organs. Mammals however, are very limited in this sense. We want to examine the relationship between nerves and regenerating tissues. Previous research has shown that nerves are both necessary and sufficient for regeneration. Neuropeptides serve as good candidate signals between nerves and tissues. In zebrafish, the Cart3 neuropeptide is present in sensory neurons and after fin fold amputation, the expression of Cart3 is upregulated. We have demonstrated that introducing additional Cart3 into larval fish increases the rate at which they are able to regenerate. We are currently working to uncover the mechanism by which Cart3 is able to enhance regeneration. We hypothesize that Cart3 enhances cell proliferation within the regeneration blastema. Another possibility is that it may work in combination with leptin (a known inducer of CART) to promote tissue remodeling. We have additionally used CRISPR-Cas9 to make several lines of mutated fish in other potential nerve-derived signals (nrg1 and agr1) for which we are testing their regenerative phenotypes.

Document Type

Poster

Share

COinS
 
Apr 2nd, 8:50 AM

Nerve to Tissue Signaling Controlling Zebrafish Regeneration

The ability to regenerate is of high variability among animals. Model organisms such as the zebrafish (Danio rerio ) are exemplary in their ability to grow back limbs and organs. Mammals however, are very limited in this sense. We want to examine the relationship between nerves and regenerating tissues. Previous research has shown that nerves are both necessary and sufficient for regeneration. Neuropeptides serve as good candidate signals between nerves and tissues. In zebrafish, the Cart3 neuropeptide is present in sensory neurons and after fin fold amputation, the expression of Cart3 is upregulated. We have demonstrated that introducing additional Cart3 into larval fish increases the rate at which they are able to regenerate. We are currently working to uncover the mechanism by which Cart3 is able to enhance regeneration. We hypothesize that Cart3 enhances cell proliferation within the regeneration blastema. Another possibility is that it may work in combination with leptin (a known inducer of CART) to promote tissue remodeling. We have additionally used CRISPR-Cas9 to make several lines of mutated fish in other potential nerve-derived signals (nrg1 and agr1) for which we are testing their regenerative phenotypes.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.